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Vectors can be used to carry both RNA and DNA sequences. They are often used in laboratory work for cloning and as a means to move genes into other organisms. Depending on the vector used, they can carry the gene of interest within the cell or between cells. The advantages of vectors over DNA fragments include:
Vectors may be manipulated more easily than isolated DNA fragments.
Vectors are passed between cells more easily than DNA fragments.
Vectors may be used to carry genes into different organisms.
Vectors are available in many different size classes. These sizes may range from tens to thousands of base pairs in length. The size of the vectors used in the study will depend on the size of the desired fragment. Several vectors are available and the one used will depend on the size of the fragment and on the destination of the vector. Vector manufacturers provide vectors in a plasmid format, where the vector is inserted into a bacterial plasmid that is then used to transform bacteria. Vectors are available in many different sizes and formats.
Vectors are highly stable and can be stored for many years, even at -80°C. The most commonly used vectors are available from several different companies. The most common vector is pUC18. This is a small plasmid that includes the origin of replication. The pUC18 also includes a gene that allows transformation of bacteria. The other more common vectors are larger, often named after the original researcher that first discovered them. These vectors are generally much more stable and have a greater capacity for carrying genes. For example, the plasmid pGEM-7Z from Promega is a 7,650 bp vector with a capacity of 40 kb. This vector is commonly used for sequencing.
You can purchase vectors from different companies. Purchase ready vectors include pre-packaged compatible bacterial cells. These are a great option for quick easy cloning. If you are not looking for a pre-packaged cell, you can purchase raw pUC18 plasmids from companies such as VBC Biomedical (
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